RESUMO
The antimutagenic activities of the oils obtained from leaves and fruits of Fagus orientalis have been shown in experiments with spontaneous and mutagen- and ageing-induced variability. The aberrations of chromosomes in the meristematic cells of the Allium cepa L., Vicia faba L., Triticum aestivum L., and marrow cells of Vistar rats as well as Arabidopsis thaliana gene mutations have been mobilized as experimental tests.
Assuntos
Antimutagênicos/farmacologia , Arabidopsis/efeitos dos fármacos , Medula Óssea/metabolismo , Fagus/química , Meristema/efeitos dos fármacos , Óleos/farmacologia , Cebolas/efeitos dos fármacos , Triticum/efeitos dos fármacos , Vicia faba/efeitos dos fármacos , Animais , Antimutagênicos/química , Arabidopsis/química , Arabidopsis/genética , Medula Óssea/química , Medula Óssea/efeitos dos fármacos , Clorofila/análise , Clorofila/química , Aberrações Cromossômicas/efeitos dos fármacos , Cromossomos/efeitos dos fármacos , Frutas/química , Meristema/química , Meristema/genética , Metilnitrosoureia/efeitos adversos , Mutagênicos/efeitos adversos , Nitrosoguanidinas/efeitos adversos , Óleos/química , Cebolas/química , Cebolas/genética , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Folhas de Planta/química , Ratos , Ratos Wistar , Triticum/química , Triticum/genética , Vicia faba/química , Vicia faba/genéticaRESUMO
Two 8-month-old and two 4-month-old male beagle dogs received 250 ml of 150 microgram/ml solution of N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG) and 2% Tween 60 mixed with a pellet diet twice a day for 8 months as the same methods used for mongrel dogs in our first report [Juntendo Medical Jouranl 19, 579-583 (1973)]. Gastric carcinomas with distant lymph nodes metastases occurred in three beagle dogs except for one died from anesthesia at the endoscopy. Metastases to the liver were observed in two beagles. In the most long-lived beagles, peritonitis carcinomatosa with ascites and metastases to the liver, lungs, bones, and skin were found. Main gastric tumors were located at the subcardia in two dogs (elevated tumor in dog No. 6, ulcerated tumor in dog No. 8), but in dog No 7 at the angulus (ulcerated tumor). Histologically, carcinomas were composed of poorly differentiated adenocarcinoma, signet-ring cell carcinoma, tubular adenocarcinoma, and undifferentiated adenocarcinoma. In all of three dogs which developed adenocarcinoma of the stomach, Stewart's criteria were completely satisifed. Using our methods the target organ is limited only to the stomach, without any sarcomatous lesion of the intestines.
Assuntos
Adenocarcinoma/induzido quimicamente , Nitrosoguanidinas/efeitos adversos , Neoplasias Gástricas/induzido quimicamente , Adenocarcinoma/patologia , Administração Oral , Animais , Cães , Masculino , Metástase Neoplásica , Neoplasias Experimentais/induzido quimicamente , Neoplasias Experimentais/patologia , Nitrosoguanidinas/administração & dosagem , Neoplasias Gástricas/patologiaRESUMO
N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG), or its derivatives N-hexyl-N'-nitro-N-nitrosoguanidine (HNNG) and 1,6-bis(N'-nitro-N-nitrosoguanidinyl)-n-hexane (HxBNNG) were given to newborn ICR/JCL mice by a single subcutaneous injection in 1% gelatin suspension. In an acute toxicity study, the maximum tolerated dose of MNNG, HNNG or HxBNNG was 62 microgram/g, 555 microgram/g, or 500 microgram/g of body weight, respectively. In a chronic study, a single subcutaneous injection of MNNG to newborn mice at a dose of 62, 31, or 3 microgram/g of body weight induced tumors of the lung and hemangioendotheliomas in both sexes, and tumors of the liver in males. Other pathologic findings, such as deformities of the spine and alopecia of the skin, were frequently observed. The incidences of tumors in each group were clearly dose related. HNNG and HxBNNG were not tumorigenic within the observation period.
